孟江洪

　　被sci收录的学术刊物发表论文41篇，参编著作8部。

　　1、开发快速检测和鉴定致病微生物的新技术 采用pcr，即时pcr（real time pcr）和molecular beacon技术检测大肠杆菌，李斯特菌和弯曲杆菌。准确率达100%。检测时间同常规方法比较，缩短了十倍以上。 开发了ram （ramification amplification）技术。极大地简化了检测过程，更加适用于食品生产过程中致病菌的快速检测。 采用pfge（pulsed-field gel electrophoresis）分型技术，提高了分辨率，有力地促进了流行病学调查，有效地预防和防止食源性疾病爆发的进一步扩散。 采用新兴的dna微阵列（dna micro array）技术检测致病菌。这项技术可以同时鉴定所有食源性致病菌。

　　2、系统揭示了食源性致病菌抗药性的产生、发展及传播 细菌的抗药性是当前世界公共卫生的一大难题，直接影响到全人类的健康。抗药性的产生涉及到多种复杂因素。除了治病过程抗生素大量滥用之外，动物生产中的大量使用抗生素也是一个加速产生抗药性的重要因素。我们在研究食源性致病菌的抗药性方面做了大量工作。首先确定了多数从食用动物和动物性食品中分离出来的细菌已经产生抗菌性。有的菌株能抵抗所有临床上用来治病的抗生素，随后，我们用分子生物学手段进一步揭示了抗药机理和传播途径。这些研究发表在国际上最具影响的公共卫生刊物上，如象《新英格兰医学杂志》（new england journal of medicine），影响因子34.8。这期刊还为此发了编者案。

　　3、揭示了弯曲杆菌的分子致病机理 同其他食源性肠道致病菌比较，弯曲杆菌有很多特异性。也是一种较难培养的一种细菌。我们基本明确了这种致病菌的致病过程。采用组织培养，动物模型和分子生物学手段，掌握了细菌在粘附、发育及侵入肠道表面的遗传学和分子生物学基础。这项研究为开发新的鉴定方法和预防疫苗奠定了基础。

　　4、目前在研项目 检测食品中大肠杆菌的新技术，美国农业部，2002年-2005年； 鸡肉中弯曲杆菌的耐药性，美国农业部，2002年-2007年； 弯曲杆菌的耐药机理，食品药物管理局，2003年-2006年； 食品中大肠杆菌0157：h7采样及快速检测，马里兰工业协作基金，2004年-2005年； 新鲜水果和蔬菜中肠道病毒的快速检测，食品药物管理局，2004年-2007年； 多项耐药沙门氏菌的dna微阵列分析，马里兰农业实验站，2005年-2006年。

　　5、主要论文

　　（1）zhao, s., d. white, b. ge, s. ayers, s. friedman, l. english, d. wagner, s. gains, and j. meng, 2001. antibiotic resistance integrons in shiga toxin-producing escherichia coli. appl. environ. microbil. 67:1558-1564.

　　（2）white, d.g., s. zhao, r. sudler, s. ayers, s. friedman, s. mcdermott, s. chen, d. wagner, and j. meng. 2001. the isolation of antimicrobial-resistant salmonella from retail ground meat. n. england j. med. 345:1147-1154.

　　（3）cui, s., j. meng, and a.a. bhagwat. 2001. availability of glutamate and arginine during acid-challenge determines cell density-dependent survival phenotype of escherichia coli. appl. environ. microbil. 67:4914-4918.

　　（4）zhao, s., d. g. white, p.f. mcdermott, s. friedman, l. english, s. ayers, j. meng, j.j maurer, r. holland and r.d. walker. 2001. identification and expression of cephamycinase blacmy genes in escherichia coli and salmonella isolated from food animals and ground meats. antimicrob agents chemother. 45:3647-3650.

　　（5）zhao, c., b. ge, j.de villena, r. sudler, e. yeh, s. zhao, d. g. white, d. wagner, and j. meng. 2001. prevalence of campylobacter, escherichia coli and salmonella in retail chicken, turkey, pork, and beef from the greater washington dc area. appl. environ. microbil. 67:5431-5436.

　　（6）schroeder, c.m., c. zhao, c. debroy, j. torcolini, s. zhao, d.g. white, d.d. wagner, r.d. walker, and j. meng. 2002. antimicrobial resistance among escherichia coli o157 isolated from humans, cattle, swine, and food. appl. environ. microbil. 68:576-581.

　　（7）ge, b., c. larkin, s. ahn, m. jolley, m. nasir, r. hall, and j. meng. 2002. identification of escherichia coli o157:h7 and other enterohemorrhagic serotypes by strand displacement amplification and fluorescent polarization. mol. cellular probe. 16:85-92.

　　（8）ge, b., s zhao, r. hall, and j. meng. 2002. pcr-elisa for detecting shiga toxin- producing escherichia coli in food. microbes infect. 4:285-290.

　　（9）white, d.g., s. zhao s, p.f. mcdermott, s. ayers, s. gaines, s. friedman, d.d. wagner, j. meng, d. needle, m. davis, c. debroy. 2002. characterization of antimicrobial resistance among escherichia coli o111 isolates of animal and human origin. microb. drug resist. 8:139-46.

　　（10）simjee, s., d. g. white, d. d. wagner, j. meng, s. qaiyumi, s. zhao, and p. f. mcdermott. 2002. identification of vat(e) in enterococcus faecalis isolates from retail poultry and its transferability to enterococcus faecium. antimicrob agents chemother 46:3823-28.

　　（11）schroeder, c. m. , j. meng, s. zhao, c. debroy, j. torcolini, c. zhao, p. f. mcdermott, d.d. wagner, r. d. walker, and d. g. white. 2002. virulence genes and antimicrobial resistance among escherichia coli o26, o103, o111, o128 and o145. emerging infect. dis. 8:1409-14.

　　（12）ge, b., s. bodies, r.d. walker, d.g. white, s. zhao, p. f. mcdermott, and j. meng. 2002. comparison of etest and agar dilution for antimicrobial susceptibility testing of campylobacter isolated from retail meats. j antimicrob chemother. 50:487-494.

　　（13）simjee s, d.g. white, j meng, d.d. wagner, s. qaiyumi, s. zhao, j.r. hayes, and p.f. mcdermott. 2002. prevalence of streptogramin resistance genes among enterococcus isolates recovered from retail meats. j antimicrob chemother. 50:6,877-882.

　　（14）schroeder, c. m., d. g. white, b. ge, y. zhang, s. zhao, p. f. mcdermott, j. meng. 2003. antimicrobial susceptibility of echerichia coli isolated from retail meats. intl. j. food microbiol. 85:197-202.

　　（15）ge, b., w. girard, d. g. white, p. f. mcdermott, s. zhao, r. d. walker, and j. meng. 2003. antimicrobial-resistant campylobacter isolated from retail raw meats. appl. environ. microbil. 69:3005-3007.

　　（16）cui, s., c. m. schroeder, d.y. zhang, j. meng. 2003. rapid sample preparation method for pcr-based detection of escherichia coli o157:h7 in ground beef. j. appl. microbiol. 95:129-134.

　　（17）senkel, i.a. jr., b. jolbitado, y. zhang, d. g.white, s. ayers, j. meng. 2003. isolation and characterization of escherichia coli recovered from apple cider and the cider production environment. j. food prot. 66:2237?c2244

　　（18）chen, s., s. zhao, d. g. white, c. m. schroeder, p. f. mcdermott, h. yang, s. ayers, j. meng. 2004. characterization of multiple-antimicrobial-resistant salmonella serovars isolated from retail meats. appl. environ. microbil. 70: 1-7.

　　（19）foley, s.l., s. simjee, j. meng, d.g. white, p.f. mcdermott, and s. zhao. 2004. evaluation of molecular typing methods for escherichia coli o157:h7 isolates from cattle, food, and humans. j. food prot. 67:651-657.

　　（20）yang, h., s. chen, d.g. white, s. zhao, r. walker, p. mcdermott and j. meng. 2004. characterization of multiple-antimicrobial-resistant escherichia coli isolated from chicken and swine in china. j. clin. microbil. 42:3483-3489.

　　（21）liming, sh, y. zhang, j. meng, and aa, bhagwat. 2004. detection of listeria monocytogenes in fresh produce using molecular beacon ?c real-time pcr technology. j. food sci. 69:240-245.

　　（22）chen, s., s. zhao, d. g. white, p. f. mcdermott, s. ayers, j. meng. 2005. a dna microarray for identification of virulence and antimicrobial resistance genes in salmonella serovars and escherichia coli mol. cellular probe. 19:195-201.

　　（23）zhao, s, j.j. maurer, s. hubert, j.f. de villena, p.f. mcdermott, j. meng, s. ayers, l. english, d.g. white. 2005. fluoroquinolone resistance of escherichia coli from diseased chicken in north georgia. vet. microbiol. 107:215-224.

　　（24）cui, s., b. ge, j. zheng, j. meng. 2005. prevalence and characterization of campylobacter spp and salmonella serovars in retail organic chickens of maryland. appl. environ. microbil. 71: 4108-4111.

　　（25）ge, b., p. mcdermott, d.g. white, and j. meng. 2005. the role of efflux pumps and gyrase a gene mutation on fluoroquinolone resistance in campylobacter jejuni/coli. antimicrob agents chemother. 19:195-201.

　　（26）singh, r., c. m. schroeder, j. meng, d. g. white, h. yang, p.f. mcdermott, d. wagner, s. simjee, c. debroy, and s. zhao. 2005. detection and characterization of antimicrobial resistance and class i integron in shiga toxin-producing escherichia coli recovered from humans and food animals. j antimicrob chemother. 56: 216-219

　　（27）ge, b., w. girard, s. zhao, j. meng. 2006. genotyping of campylobacter app. from retail meats using ribotyping and pulsed-filed gel electrophoresis. j. appl. microbiol. 100:167-174.