张守涛

　　主持的科研项目

 

　　（1）国家“十二五”国家“重大新药创制”科技重大专项滚动支持课题《 纤溶/抑制血小板聚集双功能溶栓药物研究》。

 

　　（2）国家“十一五”国家“重大新药创制”科技重大专项《 纤溶/抑制血小板聚集双功能溶栓药物研究》。

 

　　（3）国家自然科学基金《涡虫副胚层干细胞特异性microRNA的筛选及其在再生中的作用研究》。

 

　　（4）国家自然科学基金《miR-8和Let-7a调控Wnt信号通路对涡虫再生的影响及机制研究研究》。

 

　　（5）国家自然科学基金会议资助《第十届国际生物技术、金属络合物第十届国际生物技术、金属络合物和催化学术研讨会》

 

　　（6）第三批国家博士后基金特别资助《涡虫再生相关miRNA功能研究》

 

　　（7）河南省开放合作项目《靶向PD-1全人源抗体药物研究》

 

　　（8）河南省创新人才支持计划项目《涡虫再生相关miRNA调控网络及机制研究》

 

　　（9）郑州市科技攻关项目《溶栓/抗凝双功能溶栓药物生产工艺研究》

　　发表的主要论文

 

　　（1）Yun-Fei Qin, Jin-Mei Zhao, Zhen-Xia Bao, Zhao-Yu Zhu, Jia Mai, Yi-Bo Huang, Jian-Biao Li, Ge Chen, Ping Lu, San-Jun Chen, Lin-Lin Shu, Hui-Min Fang, Ji-Ke Lu, Yi-Zhe Zhang and Shou-Tao Zhang*. Identification of small non-coding RNAs in the planarian Dugesia japonica via deep sequencing. Genomics. 2012, 99(5): 315-21

 

　　（2）Qing-Nan Tian, Yun-Fei Qin, Zhen-Xia Bao, Ping Lu, San-Jun Chen, Feng Liang, Fang Wang, Shou-Tao Zhang*, Yi-Zhe Zhang. Differential expression of microRNA Patterns in Planarian Normal and Regenerative Tissues. Molecular Biology Reports. 2012. 39(3): 2653–2658

 

　　（3）Xue-Mei Huang, Qing-Nan Tian, Zhen-Xia Bao, Yun-Fei Qin, San-Jun Chen, Ping Lu, Xiao-Ling Zhang, Yi-Zhe Zhang and Shou-Tao Zhang*. Cloning and Identification of MicroRNAs in Earthworm (Eisenia fetida). Biochem Genet, 2012, 50(1): 1-11.

 

　　（4）Yun-Fei Qin, Hui-Min Fang, Qing-Nan Tian, Zhen-Xia Bao, Ping Lu,Jin-Mei Zhao, Jia Mai, Zhao-Yu Zhu, Lin-Lin Shu, Li Zhao, San-Jun Chen,Feng Liang, Yi-Zhe Zhang, Shou-Tao Zhang*. Transcriptome profiling and digital gene expression by deep-sequencing in Normal/Regenerative Tissues of Planarian Dugesia japonica. Genomics, 2011, 97(6): 364-371.

 

　　（5）Zhang ST, Fang HM, Zhao L, Tian QN, Qin YF, Lu P, Chen SJ, Bao ZX, Liang F. Co-overexpression of PpPDI enhances secretion of ancrod in Pichia pastoris. Appl Biochem Biotechnol. 2011, 164(7): 1037-47.

 

　　（6）Hui-Min Fang, Li Zhao, Ping Lu, San-Jun Chen, Zhen-Xia Bao, Yun-Fei Qin, Zhao-Yu Zhu, Jin-Mei Zhao, Jia Mai, Shou-Tao Zhang*. Modular Design, expression and Characterization of Novel Bifunctional Mutants of Fibrolase with Combined Platelet Aggregation-Inhibition and Fibrinolytic Activity. Protein Journal, 2011, 30(4): 247-252.

 

　　（7）Zhang ST, Lu P, Qin YF, Chen SJ, Guo AG. Cloning and identification of a novel P-II class snake venom metalloproteinase from Gloydius halys. Appl Biochem Biotechnol. 2010, 162(5): 1391-402.

 

　　（8）Jing Shi, Shou-Tao Zhang, Xiao-Juan Zhang, et al. expression, purification, and activity identification of Alfimeprase in Pichia pastoris. Protein expression and Purification, 2007, 54(2): 240-246.

 

　　（9）Shou-Tao Zhang, Jing Shi, Jing Zhao, Ya-Fei Qi and Ai-Guang Guo*. expression of Soluble and Functional Snake Venom Fibrinolytic Enzyme Fibrolase Via the Co-expression of DsbC in Escherichia coli. Protein and Peptide Letters, 2006, 13(6): 559-563.

 

　　（10）张守涛, 史婧, 郭蔼光. 重组蛇毒纤溶酶Fibrolase的复性. 过程工程学报. 2007, 7(4): 761-766.