主持课题：

 

　　1.       国家自然科学基金（81273228）：结核分枝杆菌耐药相关抗原CTL表位的鉴定及多价疫苗的免疫活性研究，起止年月：2013年～2016年.

 

　　2.       “十二五”科技部“重大新药创制”重大专项（2012ZX09103301-023）：新型抗肿瘤多肽药物AS16的研制，起止年月：2012年～2015年

 

　　3.       国家自然科学基金（30872381）：食管癌高表达抗原COX-2和MAGE-4的HLA-A多等位基因广谱CTL表位的筛选和鉴定，起止年月：2009年～2011年.

 

　　4.       河南省杰出人才计划项目（084200510014）：结核病抗原肽表位的筛选和多价疫苗的研制. 起止年月：2008年～2010年

　　发表的主要论文：

 

　　1.       Shi RR, Liu J, Zou Z, Qi YM, Zhai MX, Zhai WJ, Gao YF*. The immunogenicity of a novel cytotoxic T lymphocyte epitope from tumor antigen PL2L60 could be enhanced by 4-chlorophenylalanine substitution at position 1. Cancer Immunol Immunother. 2013, 62(11):1723-32.

 

　　2.       Haili Wang, Xi Chen, Yanping Chen, Lei Sun, Guodong Li, Mingxia Zhai, Wenjie Zhai, Qiaozhen Kang, Yanfeng Gao*, Yuanming Qi*. Antitumor activity of novel chimeric peptides derived from cyclinD/CDK4 and the protein transduction domain 4. Amino Acids, 2013, 44(2):499-510.

 

　　3.       Ji Z, Shi X, Liu X, Shi Y, Zhou Q, Liu X, Li L, Ji X, Gao Y, Qi Y, Kang Q. The membrane-cytoskeletal protein 4.1N is involved in the process of cell adhesion, migration and invasion of breast cancer cells. Exp Ther Med. 2012, 4(4):736-740.

 

　　4.       Yahong Wu, Yanfeng Gao*, Yujie He, Ranran Shi, Mingxia Zhai, Zongyin Wu, Meng Sun, Wenjie Zhai, Xi Chen, Yuanming Qi*. A novel cytotoxic T lymphocyte epitope analogue with enhanced activity derived from cyclooxygenase-2. Scand J Immunol. 2012, 76(3):278-85.

 

　　5.       Fei Chen, Mingxia Zhai, Yuhuang Zhu, Yuanming Qi, Wenjie Zhai, Yanfeng Gao*. In vitro and in vivo identification of a novel cytotoxic T lymphocyte epitope from Rv3425 of Mycobacterium tuberculosis. Microbiology and Immunology. 2012, 56(8):548-53.

 

　　6.       Wei Liu, Mingxia Zhai, Zongyin Wu, Yuanming Qi, Yahong Wu, Chao Dai, Meng Sun, Lu Li, Yanfeng Gao* Identification of a novel HLA-A2-restricted cytotoxic T lymphocyte epitope from cancer-testis antigen PLAC1 in breast cancer. Amino Acids. 2012, 42(6):2257-65.

 

　　7.       Zongyin Wu, Yanfeng Gao*, Yahong Wu, Wei Liu, Meng Sun, Mingxia Zhai, Yuanming Qi, Yong Ye. Identification of a novel CD8+ T cell epitope derived from cancer-testis antigen MAGE-4 in oesophageal carcinoma. Scand J Immunol. 2011, 74:561-567.

 

　　8.       Yu-huang Zhu, Yan-feng Gao*, Fei Chen, Wei Liu, Ming-xia Zhai, Wen-jie Zhai, Yuan-ming Qi*, Yong Ye. Identification of novel T cell epitopes from efflux pumps of Mycobacterium tuberculosis. Immunology Letters. 2011, 140(1-2):68-73.

 

　　9.   Jun-wei Zhao, Zhan-qiang Sun, Hong-guo Yang, Chong-zhen Zhang and Xiao-li Yu, Zi-lu Wen, Yan-feng Gao, Xiao-kui Guo, Yuan-ming Qi, Shu-lin Zhang. Cloning, expression and immunological evaluation of a short fragment from Rv3391 of Mycobacterium tuberculosis. Annals of Microbiology, 2011, 61(2):345-353.

 

　　10.   Hong Lv, Yan-feng Gao*, Ya-hong Wu, Ming-xia Zhai, Lu Li, Yu-huang Zhu, Wei Liu, Zong-yin Wu, Fei Chen, Yuan-ming Qi. Identification of a novel cytotoxic T lymphocyte epitope from CFP21, a secreted protein of Mycobacterium tuberculosis. Immunology Letters. 2010, 133(2):94-8.

 

　　11.   Yanfeng Gao*, Kun Chen, Bin Zhang, Xiang Li, Lu Chen, Yuanjun Li, Xiangjiao Jia, Yongxing Lei, Zhongyi Yan, Liangliang Kong, Nan Wang, Wei Liu, Yuanming Qi. Antioxidant and free radical scavenging activity of the extracellular death factor in Escherichia coli. Peptides, 2010, 31(10):1821-1825.

 

　　12.   Dong-dong Wu, Yan-feng Gao, Li-xiang Chen*, Yuan-ming Qi*, Qiao-zhen Kang, Hai-li Wang, Lin-yu Zhu, Yong Ye, Ming-xia Zhai. Anti-tumor effects of a novel chimeric peptide on S180 and H22 xenografts bearing nude mice. Peptides. 2010, 31(5):850-864.

 

　　13.   Yan-feng Gao, Zhan-qiang Sun, Feng Qi, Yuan-ming Qi*, Ming-xia Zhai, Hui-ping Lou, Li-xiang Chen, Yong-xin Li, Xian-yuan Wang. Identification of a new broad-spectrum CD8+ T cell epitope from over-expressed antigen COX-2 in esophageal carcinoma. Cancer Letters, 2009, 284(1):55-61.

 

　　14.   Zhang SL, Zhao JW, Sun ZQ, Yang EZ, Yan JH, Zhao Q, Zhang GL, Zhang HM, Qi YM, Wang HH, Sun QW. Development and evaluation of a novel multiple-antigen ELISA for serodiagnosis of tuberculosis. Tuberculosis (Edinb). 2009, 89(4):278-284.

 

　　15.   Hui-ping Lou, Yan-feng Gao*, Ming-xia Zhai, Yuan-ming Qi*, Li-xiang Chen, Hong Lv, Ji-bing Yu, Yong-xin Li. A novel peptide from α5 helix of Asterina pectinifera cyclin B conjugated to HIV-Tat49–57 with cytotoxic and apoptotic effects against human cancer cells. Bioorganic & Medicinal Chemistry Letters, 2008, 18(16):4633-4637.

 

　　16.   Yan-feng Gao, Ming-xia Zhai, Wei-xia Liu, Xin Liu, Ye Yuan, Yuan-ming Qi*, Rui Wang*. Structure-activity relationships of the dimeric analogues of endomorphin-2 with different lengths of spacers. Protein & Peptide Letters, 2008, 15(3):275-279.

 

　　17.   Lu GF, Tang FA, Zheng PY, Yang PC, Qi YM. Entecavir up-regulates dendritic cell function in patients with chronic hepatitis B. World J Gastroenterol. 2008, 14(10):1617-1621.

 

　　18.   Zheng PY, Zhang DY, Lu GF, Yang PC, Qi YM, Wang BS. Effects of lamivudine on the function of dendritic cells derived from patients with chronic hepatitis B virus infection. World J Gastroenterol. 2007, 13(34):4641-5.

 

　　19.   Li-xiang Chen, Yuan-ming Qi*, Rui Wang*, Xin Duan, Yan-feng Gao, Ding-jian Yang. Roles of nitric oxide synthase inhibitor on antinociceptive effects of μ-Opioid agonist in mice. Protein & Peptide Letters, 2006, 13(10): 993-997.

 

　　20.   Yan-feng Gao, Xin Liu, Wei-xia Liu, Yuan-ming Qi, Xue-feng Liu, Rui Wang*. Opioid receptor binding and antinociceptive activity of the analogues of endomorphin-2 and morphiceptin with phenylalanine mimics in the position 3 or 4. Bioorganic & Medicinal Chemistry Letters, 2006, 16(14):3688-3692.

 

　　21.   Wang JX, Liu GH, Fan YZ, Liu QL, Zhou J, Zhang DY, Qi YM. Hepatobiliary Pancreat Dis Int. 2006, 5(3):422-427.

 

　　22.   Zheng PY, Tang FA, Qi YM, Li J. Association of peptic ulcer with increased expression of Lewis antigens, but not vacuolating cytotoxin activity or babA2 gene status, in Helicobacter pylori strains from China. Chin J Dig Dis. 2006, 7(1):61-65.

 

　　23.   Qi YM, Yang DJ, Duan X, Yang F, Li SR, Shen JM, Wang R. Endomorphins inhibit contractile responses of rat thoracic aorta rings induced by phenylephrine and angiotensin II in vitro. Acta Pharmacol Sin. 2002, 23(1):40-44.

　　授权专利：

 

　　1.       祁元明，吴亚红，高艳锋，陈飞，孙萌，韩艳林，时冉冉，陈艳平. MTA1来源的抗肿瘤CTL表位肽及其应用. 国家发明专利授权号：ZL201110138635.4; ZL201310112954.7; ZL201310112755.6; ZL201310112763.0.

 

　　2.       祁元明，陈飞，高艳锋，朱宇皇，吴亚红，陈艳平，韩艳林. 结核耐药相关外排蛋白来源抗结核CTL表位肽及其应用. 国家发明专利授权号：ZL201110138646.2; ZL201310101212.4; ZL 201310101237.4; ZL201310101482.5.

 

　　3.       祁元明，朱宇皇，高艳锋，吕虹，翟明霞，陈飞. 结核分枝杆菌抗原限制性表位肽. 国家发明专利授权号：ZL201010030225.3.

 

　　4.       祁元明，吴宗胤，高艳锋，吕虹，刘伟，吴亚红，李璐. MAGE-4抗肿瘤CTL表位肽及其应用. 国家发明专利授权号：ZL201010212521.5.

 

　　5.       祁元明，吕虹，高艳锋，朱宇皇，刘伟，李璐，吴亚红，吴宗胤. 抗结核CTL表位肽. 国家发明专利授权号：ZL200910065403.3.

 

　　6.       高艳锋，陈艳平，祁元明，刘蓓媛. 具有抗肿瘤活性的PD-L1亲和肽及其应用. 国家发明专利授权号：ZL201310283767.5.

 

　　7.       高艳锋，刘伟，祁元明，吕虹，吴宗胤，李璐，吴亚红. PLAC1抗肿瘤CTL表位肽及其应用. 国家发明专利授权号：ZL201010205684.0; ZL201110423341.6.

 

　　8.       高艳锋，翟明霞，刘伟，祁元明，吕虹，吴亚红，代怊，康巧珍. PIWIL2抗肿瘤CTL表位肽及其应用. 国家发明专利授权号：ZL201010276917.6.

 

　　9.       翟明霞，时冉冉，高艳锋，祁元明，刘伟. PL2L60来源的CTL表位肽及其应用. 国家发明专利授权号：ZL201210001557.8.

 

　　10.   祁元明，孙战强，陈鲤翔，李永欣，祁峰，娄慧萍，李静静，胡红敏. 具有人群广谱性的抗肿瘤CTL表位肽. 国家发明专利授权号：ZL200710054307.X.

 

　　11.      康巧珍，李黎，刘鑫，汲振余，翟文杰，李国栋，高艳锋，祁元明. Th1细胞因子免疫调节多肽及其应用. 国家发明专利授权号：ZL201210004099.3.