研究领域和方向

　　1、动物细胞培养过程及其生物反应器工程

　　流体混合与气液传质、细胞与气泡相互作用、细胞与湍流涡旋相互作用、放大与缩小等生物反应器工程基本科学问题；

　　高密度培养过程中动物细胞物质能量代谢调控与目的产物表达、加工机制；

　　动物细胞培养过程及其生物反应器设计优化、放大缩小和强化操作；

　　动物细胞生理状态与病毒感染、复制的关系以及病毒疫苗高效生产关键技术；

　　动物细胞无血清无蛋白培养基高效开发与设计优化。

　　2、人体干细胞生物工程

　　体外培养环境对人体干细胞生长、生物学功能和基因表达的作用及其机制；

　　人体干细胞体外培养、扩增和定向诱导分化技术及其生物反应器工程。

　　3、人体组织工程

　　组织工程中的流体力学和物质传递现象；

　　生物材料与干细胞的相互作用；

　　工程化组织体外构建技术及其生物反应器工程。

　　近年主持和承担的主要科研项目：

　　1. 动物细胞表达产品中试放大及分离纯化技术平台，“重大新药创制”国家科技重大专项（2009.01－2011.12），课题编号：2009ZX09306－007。

　　2. 哺乳动物细胞大规模培养及药物制备关键技术，“重大新药创制”国家科技重大专项（2009.01－2011.12），课题编号：2009ZX09503-013。

　　3. 动物细胞大规模无血清培养及表达产品制备技术，国家科技支撑计划重点项目（2008.01－2010.12），课题编号：2008BAI63B02。

　　4. 基于细胞培养H1N1或其它亚型流感疫苗研究，国家863计划重点项目（2010.01－2012.12），课题编号：2010AA022905。

　　近年代表性论文：

　　2012年：

　　[1]. Fei Chen, Li Fan, Jiaqi Wang, Yan Zhou, Zhaoyang Ye, Liang Zhao, Wen-Song Tan (2012) Insight into the roles of hypoxanthine and thydimine on cultivating antibody-producing CHO cells: cell growth, antibody production and long-term stability. Applied Microbiology and Biotechnology, 93: 169-178.

　　[2]. Fei Chen, Zhaoyang Ye, Liang Zhao, Xuping Liu, Li Fan, Wen-Song Tan (2012) Correlations of antibody production rate with glucose and lactate metabolism in Chinese hamster ovary cells. Biotechnology Letters, 34: 425-432.

　　[3]. Zesong Wang, Xuhui Ma, Liang Zhao, Li Fan, Wen-Song Tan (2012) expression of anti-apoptotic 30Kc6 gene inhibiting hyperosmotic pressure-induced apoptosis in antibody-producing Chinese hamster ovary cells, Process Biochemistry, 47: 735–741.

　　[4]. Zesong Wang, Xuhui Ma, Li Fan, Won Jong Rhee, Tai Hyun Park, Liang Zhao, Wen-Song Tan (2012) Understanding the mechanistic roles of 30Kc6 gene in apoptosis and specific productivity in antibody-producing Chinese hamster ovary cells, Applied Microbiology and Biotechnology, 94(5): 1243-1253.

　　[5]. Fei Chen, Zhaoyang Ye, Liang Zhao, Xuping Liu, Li Fan, and Wen-Song Tan (2012) Biphasic addition strategy of hypoxanthine and thymidine for improving monoclonal antibody production. Journal of Bioscience and Bioengineering, 114(3): 347-352.

　　[6]. Jinli Fan, Haibo Cai, Qunliang Li, Zheng Du, Wensong Tan (2012) The effects of ROS-mediating oxygen tension on human CD34+CD38- cells induced into mature dendritic cells, Journal of Biotechnology, 158: 104-111.

　　[7]. Zheng Du, Haibo Cai, Zhaoyang Ye, Wen-Song Tan (2012) Optimization of SCF feeding regimen for ex vivo expansion of cord blood hematopoietic stem cells, Journal of Biotechnology, 164: 211-219.

　　[8]. Yi Zhang, Yan Zhang, Min Chen, Jinliang Yan, Zhaoyang Ye, Yan Zhou, Wensong Tan, Meidong Lang (2012) Surface properties of amino-functionalized poly(ε-caprolactone) membranes and the improvement of human mesenchymal stem cell behavior, Journal of Colloid And Interface Science, 368: 64-69.

　　[9]. Houyong Luo, Maiqin Chen, Xiu Wang, Yang Mei, Zhaoyang Ye, Yan Zhou, Wen-Song Tan (2012) Fabrication of centimeter-sized viable 3D tissue constructs endowed with microchannel conduits for improved tissue properties through assembling of cell-laden microbeads, Journal of Tissue Engineering And Regenerative Medicine. (Online, DOI: 10.1002/term.1554)

　　[10]. Qingchun Zhang, Ke Tan, Zhaoyang Ye, Yan Zhang, Wensong Tan, Meidong Lang (2012) Preparation of open porous polycaprolactone microspheres and their applications as effective cell carriers in hydrogel system, Materials Science and Engineering C. 32: 2589-2595.

　　[11]. Qiang Jing, Haibo Cai, Zheng Du, Zhaoyang Ye ,Wen-Song Tan (2012) Effects of agitation speed on the ex vivo expansion of cord blood hematopoietic stem/progenitor cells in stirred suspension culture, Artificial Cells, Blood Substitutes, And Biotechnology, (Early Online: 1-5 DOI: 10.3109/10731199. 2012.712043)

　　[12]. Jianyun Ge, Haibo Cai, Qunliang Li, Zheng Du, Wen-Song Tan (2012) The effects of telomerase activity and apoptosis on ex vivo expansion of cord blood CD34+ cells, Cell Proliferation, (Accepted).

　　2011年：

　　[1]. Tian-Ci Kou, Li Fan, Zhaoyang Ye, Yan Zhou, Xuping Liu, Liang Zhao, Wen-Song Tan (2011) Detailed understanding of enhanced specific productivity in Chinese hamster ovary cells at low culture temperature, Journal of Bioscience and Bioengineering, 111: 365-369.

　　[2]. Tian-Ci Kou, Li Fan, Zhaoyang Ye, Yan Zhou, Liang Zhao, Wen-Song Tan (2011) Increasing the productivity of TNFR-Fc in GS-CHO cells at reduced culture temperature, Biotechnology and Bioprocess Engineering, 16: 136-143.

　　[3]. Zesong Wang, Ju Hyun Park, Hee Ho Park, Wensong Tan, and Tai Hyun Park (2011) Enhancement of Recombinant Human EPO Production and Sialylation in Chinese Hamster Ovary Cells Through Bombyx mori 30Kc19 Gene expression. Biotechnology and Bioengineering, 108: 1634-1642.

　　[4]. Tian-ci Kou, Li Fan, Zhao-yang Ye, Yan Zhou, Xu-ping Liu, Liang Zhao, Wen-Song Tan (2011) Process analysis of reduced specific productivity of TNFR-Fc in Chinese hamster ovary cells at high cell density. Process Biochemistry, 46: 1492-1499.

　　[5]. Kun Niu，Xu Zhang, Wensong Tan, Minglong Zhu (2011) Effect of culture conditions on producing and uptake hydrogen flux of biohydrogen fermentation by metabolic flux anaylsis method, Bioresource Technology, 102: 7294-7300.

　　[6]. Zhaoyang Ye, Yan Zhou, Haibo Cai, Wensong Tan (2011) Myocardial Regeneration: Roles of Stem Cells and Hydrogels, Advanced Drug Delivery Reviews, 63: 688–697.

　　[7]. Maiqin Chen, Xiu Wang, Zhaoyang Ye, Yan Zhang, Yan Zhou, Wen-Song Tan (2011) A modular approach to the engineering of centimeter-sized bone tissue constructs using human amniotic mesenchymal stem cells-laden microcarriers Biomaterials, 32: 7532-7542.

　　[8]. Wei Wu, Zhaoyang Ye, Yan Zhou, Wen-Song Tan (2011) AICAR, a small chemical molecule, primes osteogenic differentiation of adult mesenchymal stem cells, The International Journal of Artificial Organs, 34(12): 1128-1136.

　　[9]. Jianyun Ge, Haibo Cai, Wen-Song Tan (2011) Chromosomal stability during ex vivo expansion of UCB CD34+ cells. Cell Proliferation, 44: 550-557.

　　[10]. Fei Chen, Tianci Kou, Li Fan, Yan Zhou, Zhaoyang Ye, Liang Zhao, Wen-Song Tan (2011) The Combined Effect of Sodium Butyrate and Low Culture Temperature on the Production, Sialylation, Biological Activity of an Antibody Produced in CHO Cells. Biotechnology and Bioprocess Engineering, 16: 1157-1165.

　　[11]. Yan Jinliang, Ye Zhaoyang, Chen Min, Liu Zhanzhan, Xiao Yan, Zhang Yan, Zhou Yan, Tan Wen-song, Lang Meidong (2011) Fine tuning micellar core-forming block of poly(ethylene glycol)-block-poly(ε-caprolactone) amphiphilic copolymers based on chemical modification for the solubilization and delivery of doxorubicin, Biomacromolecules, 12(7): 2562-2572.

　　[12]. Yan Jinliang, Ye Zhaoyang, Luo Houyong, Chen Min, Zhou Yan, Tan Wensong, Xiao Yan, Zhang Yan, Lang Meidong (2011) Synthesis, characterization, fluorescence labeling and cellular internalization of novel amine-functionalized poly(ethylene glycol)-block-poly (ε-caprolactone) amphiphilic block copolymers, Polymer Chemistry, 2: 1331-1340.

　　2010年：

　　[1]. Xu Zhang, Xu-Jie Duan, Wen-Song Tan (2010) Mechanism for the effect of agitation on the molecular weight of hyaluronic acid produced by Streptococcus zooepidemicus, Food Chemistry, 119: 1643-1646.

　　[2]. Kun Niu, Xu Zhang, Wen-Song Tan, Ming-Long Zhu (2010) Characteristics of fermentative hydrogen production with klebsiella pneumoniae ECU-15 isolated from anaerobic sewage sludge, International Journal of Hydrogen Energy, 35: 71－80.

　　[3]. Lixin Sun, Xu Zhang, Wensong Tan, Minglong Zhu, Ruiqiang Liu, Chunqiang Li (2010) Rheology of pyrite slurry and its dispersant for the biooxidation process, Hydrometallurgy, 104:178-185.

　　[4]. Yigang Wang, Lingfeng He, Guoqing He, Yanping Kong, Xuping Liu, Haibo Cai, Xinyuan Liu, and Wensong Tan (2010) Enhanced antitumor effect of combining interferon β with TRAIL mediated by tumor-targeting adeno-associated virus vector on A549 lung cancer xenograft, Chin J Biotechnol, 26(6): 780-788.

　　[5]. Yigang Wang, Fang Huang, Haibo Cai, Yumei Wu, Guoqing He, Wen-Song Tan (2010) The efficacy of combination therapy using adeno-associated virus-TRAIL targeting to telomerase activity and cisplatin in a mice model of hepatocellular carcinoma, Journal of Cancer Research and Clinical Oncology, 136(12): 1827-1837.

　　[6]. Xuping Liu, Liang Zhao, Yigang Wang, Xu Zhang and Wen-Song Tan (2010) Effects of calcium ion on adenovirus production with high densities of HEK293 cells, Biotechnology and Bioprocess Engineering, 15:414-420.

　　[7]. Li Fan, Liang Zhao, Zhaoyang Ye, Yating Sun, Tianci Kou, Yan Zhou and Wen-Song Tan (2010) Effect of culture temperature on TNFR-Fc productivity in recombinant glutamine synthetase-chinese hamster ovary cells, Biotechnology Letters, 32: 1239-1244.

　　[8]. Zesong Wang, Ju Hyun Park, Hee Ho Park, Wensong Tan, Tai Hyun Park (2010)  Enhancement of therapeutic monoclonal antibody production in CHO cells using 30Kc6 gene, Process Biochemistry, 45: 1852-1856.

　　[9]. Yang Mei, Houyong Luo, Qiang Tang, Zhaoyang Ye, Yan Zhou, Wen-Song Tan (2010) Modulating and modeling aggregation of cell-seeded microcarriers in stirred culture system for macrotissue engineering, Journal of Biotechnology, 150:  438–446.

　　[10]. Min Wang, Yan Zhou, Wen-Song Tan (2010) Clonal Isolation and Characterization of Mesenchymal Stem Cells from Human Amnion, Biotechnology and Bioprocess Engineering, 15: 1047-1058.

　　2009年：

　　[1]. Xuping Liu, Yigang Wang, Hongxing Niu, Xu Zhang and Wen-Song Tan（2009）The improvement of adenovirus vector production by increased expression of coxsackie adenovirus receptor, Biotechnology Letters, 31(7): 939-944.

　　[2]. Ling Feng He, Yi Gang Wang, Tian Xiao, Kang Jiang Zhang, Gong Chu Li, Jin Fa Gu, Liang Chu, Wen Hao Tang, Wen-Song Tan, Xin Yuan Liu (2009) Suppression of cancer growth in mice by adeno-associated virus vector-mediated IFN-β expression driven by hTERT promoter, Cancer Letters, 286: 196-205.

　　[3]. Kun Niu, Peng Chen, Xu Zhang, Wen-Song Tan (2009) Enhanced enzymatic hydrolysis of rice straw pretreated by alkali assisted with photocatalysis technology, Journal of Chemical Technology and Biotechnology. 84: 1240-1245.

　　[4]. Liang Zhao, Li Fan, Jiaqi Wang, Hongxing Niu, and Wen-Song Tan (2009) Responses of GS-NS0 Myeloma Cells to Osmolality: Cell Growth, Intracellular Mass metabolism, Energy metabolism, and Antibody Production. Biotechnology and Bioprocess Engineering, 14：625－632。

　　[5]. Xu-Jie Duan, Hong-Xing Niu, Wen-Song Tan, Xu Zhang (2009) Mechanism analysis of effect of oxygen on molecular weight of hyaluronic acid produced by Streptococcus zooepidemicus. Journal of Microbiology and Biotechnology. 19(3): 299-306.

　　[6]. Shi Yang, Haibo Cai, Huili Jin, Jinli Fan, and Wen-song Tan (2009) Hematopoietic Reconstitution of CD34+ cells derived from Short-term Cultured Cord Blood Mononuclear Cells. Biotechnology and Bioprocess Engineering, 14:429~435.

　　[7]. Ting Chen, Yan Zhou, Wen-Song Tan (2009) Influence of lactic acid on the proliferation, metabolism, and differentiation of rabbit mesenchymal stem cells, Cell Biology and Toxicology, 25：573-586.

　　[8]. Ting Chen, Yan Zhou, Wen-Song Tan（2009）Effects of low temperature and lactate on osteogenic differentiation of human amniotic mesenchymal stem cells, Biotechnology and Bioprocess Engineering, 14:708-715.

　　[9]. Fan Li, Liang Zhao, Yating Sun, Tianci Kou, Yan Zhou, Wen-song Tan (2009) A High-Yielding, Generic Fed-Batch Process for Recombinant Antibody Production of GS-Engineered Cell Lines, Journal of Microbiology and Biotechnology, 19(12): 1695-1702.

　　荣誉和奖励

　　1996年入选上海市曙光计划。

　　1998年入选上海市启明星计划。

　　1999－2000年获上海市政府特殊津贴。

　　2000年荣获“立达”奖教金。

　　2000年入选教育部骨干教师资助计划。

　　2001年入选教育部优秀青年教师资助计划。

　　2002年入选教育部跨世纪优秀人才培养计划。

　　授权中国发明专利：

　　1. 细胞灌注培养过程中的细胞截留分离方法及其装置，ZL 00116518.6，2003.6.25。

　　2. 用于干细胞体外培养的生物反应器系统，ZL 200310109169.2，2006.6.28。

　　3. 造血细胞连续灌注悬浮培养过程细胞沉降截留装置，ZL 200310109264.2，2006.11.29。

　　4. 一种CIK细胞体外培养方法，ZL 200310109565.5，2006.3.15。

　　5. 由脐血CD34+细胞体外诱导生成巨核细胞的方法，ZL 200310122982.3，2006.12.30。

　　6. 一种造血细胞灌注培养方法和装置，ZL 200410016212.5，2007.1.31。

　　7. 用于皮肤角质细胞体外培养和扩增的无血清培养基，ZL 200410016367.9，2006.8.6。

　　8. 双层活性皮肤体外构建的灌注式生物反应器系统，ZL 200410016651.6，2006.5.24。

　　9. 骨髓间充质干细胞的规模化制备方法，ZL 200410017587.3，2007.5.16。

　　10. 三维立体培养和诱导骨髓间充质干细胞成软骨细胞的方法，ZL 200410017588.8，2009,8,19。

　　11. 采用生物反应器制备双层活性人工皮肤组织的方法，ZL 200410017155.2，2007.6.20。

　　12. 适用于多种动物细胞大规模培养的无血清培养基，ZL 200510030198.9，2008.1.16。

　　13. 用于动物细胞大规模培养的生物反应器系统，ZL 200510030197.4，2008.3.19。

　　14. 倾斜式重力沉降活细胞截留装置，ZL 200610118405.0，2009.3.18。

　　15. 用于难浸硫化金矿生物氧化的反应器，ZL 200810037672.4。

　　16. 用于透明质酸发酵的新型反应器，ZL 200710043820.9，2010.11.17。

　　17. 适于动物细胞产品生产的无动物来源低蛋白培养基，ZL 200910053506.8，2011.1.19。

　　18. 肺炎克雷伯氏菌及其制备氢气和2,3-丁二醇的方法，ZL 200910046726.8，2011.7.13。

　　19. 适于NS0细胞大规模培养及抗体生产的无蛋白培养基，ZL 200810039305.8，2012.1.4。

　　20. 适于MDCK细胞大规模贴壁培养和单细胞悬浮培养的无血清培养基，ZL 201010022834.4，2012.3.27。

　　21. 用于造血细胞体外培养的生物反应器系统，ZL 200710044747.7，2012.5.23。

　　22. 用于骨髓间充质干细胞体外培养和扩增的无血清培养基，ZL 200710047035.0，2012.6.13。