承担和参加的主要科研项目：

　　1. 国家自然科学基金项目“VI型分泌系统（T6SS）在溶藻弧菌中调控碱性丝氨酸蛋白酶合成/分泌的分子机制”，30901113，2010-2012 (主持)

　　2. 国家自然科学基金项目“溶藻弧菌中RseA-RpoE 介导PpkA/PppA 调控群体感应和毒力的分子机制”，31372560，2014-2017(主持)

　　3. 上海市科委科技启明星人才计划项目“迟钝爱德华氏菌中基于全基因组/转录组信息的T3SS和T6SS效应蛋白的系统鉴定和作用机制研究”，13QA1401000, 2013.07-2015.06 (主持)

　　4. 农业部行业公益性专项“东海渔业资源评价与增殖养护技术研究”子课题“流行病学调查和增殖放流健康标准”，nyhyzx-201303047，2013.01-2017.12 (主持)

　　5. 农业部国家鲆鲽类产业技术体系“疾病防控岗位”，CARS-50, 2012-2016 (参加)

　　6. 国家863计划主题项目“迟钝爱德华氏菌活疫苗的产业化开发及应用”， 2013AA093101，2013.1-2017.12 (参加)

　　发表论文：

　　2009年9月1日以后的成果(通讯作者*或第一作者)

　　1. Jingfan Xiao, Qiyao Wang*, Qin Liu, Lili Xu, Xin Wang, Haizhen Wu, and Yuanxing Zhang* Characterization of Edwardsiella tarda rpoS: effect on serum resistance, chondroitinase activity, biofilm formation, and autoinducer synthetases expression, Applied Microbiology and Biotechnology, 2009, 83:151-160.

　　2. Qiyao Wang, Minjun Yang, Jingfan Xiao, Haizhen Wu, Xin Wang, Yuanzhi Lv, Lili Xu, Huajun Zheng, Shengyue Wang, Guoping Zhao, Qin Liu, Yuanxing Zhang. Genome sequence of the versatile fish pathogen Edwardsiella tarda provides insights into its adaptation to broad host ranges and intracellular niches. PLoS ONE, 2009, 17, 4(10): e7646.

　　3. Xin Wang, Qiyao Wang*, Jingfan Xiao, Qin Liu, Haizhen Wu, Lili Xu, Yuanxing Zhang*. Edwardsiella tarda T6SS component evpP is regulated by esrB and iron, and plays essential roles in the invasion of fish. Fish & Shellfish Immunology, 2009, 27(3): 469-477.

　　4. Xin Wang, Qiyao Wang*, Minjun Yang, Jingfan Xiao, Qin Liu, Haizhen Wu, and Yuanxing Zhang*. QseBC controls flagellar motility, fimbrial hemagglutination and intracellular virulence in fish pathogen Edwardsiella tarda. Fish & Shellfish Immunology. 2011, 30: 944-953

　　5. Huan Liu,?Qiyao Wang*, Qin Liu, Xiaodan Cao, Cunbin Shi,?Yuanxing Zhang*. Roles of Hfq in the stress adaptation and virulence in fish pathogen Vibrio alginolyticus and its potential application as a target for live attenuated vaccine, Applied Microbiology and Biotechnology, 2011, 91:353-364

　　6. Jingfan Xiao, Tao Chen, Qiyao Wang*, Qin Liu, Xin Wang, Yuanzhi Lv, Haizhen Wu, and Yuanxing Zhang*. Search for live attenuated vaccine candidate against edwardsiellosis by mutating virulence-related genes of fish pathogen Edwardsiella tarda . Letters in Applied Microbiology. 2011, 53(4):430-437

　　7. Yamin Wang, Qiyao Wang*, Jingfan Xiao, Qin Liu, and Yuanxing Zhang. Genetic Relationships of Edwardsiella Strains Isolated in China Aquaculture Revealed by rep-PCR Genomic Fingerprinting and Investigation of Edwardsiella Virulence Genes. Journal of Applied Microbiology, 2011, 111(6): 1337-1348 (Q2)

　　8. Huan Liu, Dan Gu, Xiaodan Cao, Qin Liu, Qiyao Wang*, Yuanxing Zhang*. Characterization of a new quorum sensing regulator luxT and its roles in the extracellular protease production, motility and virulence in fish pathogen Vibrio alginolyticus. Archives of Microbiology. 2012, 194:439-452 (Q3)

　　9. Lili Sheng, Qiyao Wang*, Qin Liu, Yuanxing Zhang Connection between Type VI Secretion System, Quorum Sensing, and Alternative Sigma Factor in Fish Pathogen Vibrio alginolyticus. Archives of Microbiology, 2012, 194(5):379-390

　　10. Yuanzhi Lv, Jingfan Xiao, Qin Liu, Haizhen Wu, Yuanxing Zhang, Qiyao Wang*. Systematic mutation analysis of two-component signal transduction systems reveals EsrA-EsrB and PhoP-PhoQ as the major virulence regulators in Edwardsiella tarda. Veterinary Microbiology. 2012, 157:190-199

　　11. Jingfan Xiao, Tao Chen, Qiyao Wang*, Yuanxing Zhang. Comparative analysis of the roles of catalases KatB and KatG in the physiological fitness and pathogenesis of fish pathogen Edwardsiella tarda. Letters in Applied Microbiology. 2012, 54, 425-432 (Q3)

　　12. Minjun Yang, Jingfan Xiao, Haizhen Wu, Yuanzhi Lv, Huajun Zheng, Qin Liu, Yuanxing Zhang, Qiyao Wang*. Edwardsiella comparative phylogenomics reveal the new intra/inter-species taxonomic relationships, virulence evolution and niche adaptation mechanisms. PLOS ONE. 2012, 7(5): e36987.

　　13. Jingfan Xiao, Xin Wang, Tao Chen, Yuanxing Zhang, Qiyao Wang*. Identification of qseEGF genetic locus and its roles in regulating hemolytic activity and invasion in fish pathogen Edwardsiella tarda. Letters in Applied Microbiology, 2012, 55(2):91-98

　　14. Huan Liu, Dan Gu, Lili Sheng, Qiyao Wang*, Yuanxing Zhang. Investigation of the roles of T6SS genes in motility, biofilm formation, and extracellular protease Asp production in Vibrio alginolyticus with modified Gateway-compatible plasmids. Letters in Applied Microbiology, 2012, 55:73-81

　　15. Yuanzhi Lv, Junyuan Zheng, Minjun Yang, Qiyao Wang*, Yuanxing Zhang. An Edwardsiella tarda mutant lacking UDP-glucose dehydrogenase shows pleiotropic phenotypes, attenuated virulence, and potential as a vaccine candidate. Veterinary Microbiology, 2012, 160: 506-512

　　16. Lili Sheng, Yuanzhi Lv, Qin Liu, Qiyao Wang*, Yuanxing Zhang. Connecting type VI secretion, quorum sensing, and c-di-GMP production in fish pathogen Vibrio alginolyticus through phosphatase PppA, Veterinary Microbiology, 2013, 162: 652-662

　　17.  Yamin Wang, Weizheng Yang, Qiyao Wang*, Yuanxing Zhang. Functional characterization of Edwardsiella tarda twin-arginine translocation system and its potential use as a biological containment for live attenuated vaccine in marine fish vaccinology. Applied Microbiology and Biotechnology, 2013, 97(8): 3543-3557

　　18. Yamin Wang, Qiyao Wang*, Minjun Yang, Yuanxing Zhang. Proteomic analysis of a twin-arginine translocation deficient mutant unravel its functions involved in stress adaptation and virulence in fish pathogen Edwardsiella tarda. FEMS Microbiology Letters, 2013, 343:145-155.

　　19. Yuanzhi Lv, Kaiyu Yin, Shuai Shao, Qiyao Wang*, Yuanxing Zhang. Comparative proteomic analysis reveals new components of the PhoP regulon and highlights a role for PhoP in the regulation of genes encoding the F1F0 ATP synthase in Edwardsiella tarda, Microbiology-SGM, 2013, 159: 1340-1351. (Q2)

　　20. Jingfan Xiao, Tao Chen, Bin Liu, Weizheng Yang, Qiyao Wang*, Jiangbo Qu, Yuanxing Zhang. Edwardsiella tarda mutant disrupted in type III secretion system and chorismic acid synthesis and cured of a plasmid as a live attenuated vaccine in turbot. Fish & Shellfish Immunology, 2013, 35: 632-641. (Q1)

　　21. Minjun Yang, Shuai Shao, Jingfan Xiao, Qiyao Wang*, Yuanxing Zhang. Phylogenetic investigation of Edwardsiella tarda with multilocus sequence typing (MLST) and pulsed field gel electrophoresis typing (PFGE) methods. Aquaculture, 2013, 410-411:79-85. (Q1)

　　22. Yamin Wang, Weizheng Yang, Qiyao Wang*, Jiangbo Qu, Yuanxing Zhang. Presenting a foreign antigen on live attenuated Edwardsiella tarda using twin-arginine translocation signal peptide as a multivalent vaccine. Journal of Biotechnology, 2013, 168:710-717(Q2)

　　23. Weizheng Yang, Lixia Wang, Lingzhi Zhang, Jiangbo Qu, Qiyao Wang*, Yuanxing Zhang. An invasive and low virulent Edwardsiella tarda esrB mutant promising as live attenuated vaccine in aquaculture. Appl Micbriol Biotechnol, 2015, 99:1765–1777 (Q1)

　　24. Shuai Shao, Qiliang Lai, Qin Liu Haizhen Wu, Jingfan Xiao, Zongze Shao, Qiyao Wang*, Yuanxing Zhang. Phylogenomics characterization of a highly virulent Edwardsiella strain ET080813T encoding two distinct T3SS and three T6SS gene clusters: propose a novel species as Edwardsiella anguillarum sp. nov. Systematic and Applied Microbiology, 2015, 38: 36-47. (Q2)

　　专利：

　　1 刘琴, 张元兴, 杨朝, 王启要. 细菌表面展示新系统、方法及应用. ZL 101182516, 2008.05.21

　　2 刘琴, 张元兴, 杨朝, 王启要. 新型细菌表面展示系统、方法及应用. ZL 101195823, 2008.06.11

　　3 张元兴, 王鑫, 王启要, 刘琴. 一种迟钝爱德华氏菌野生毒株的无标记基因缺失减毒突变株及其应用, ZL 200910052707.6

　　4 王启要, 张元兴, 刘欢, 刘琴. 一种溶藻弧菌减毒株的无标记基因缺失减毒突变株、相关制剂其应用, 中国发明专利. 申请号：ZL 201110266453.5

　　5 王启要, 陈涛, 王鑫, 肖婧凡, 刘琴, 张元兴. 迟钝爱德华氏菌野生毒株的无标记基因缺失减毒株、相关制剂及其应用, ZL 201010541646.2

　　6 刘琴，张元兴，周凌云，王启要. 一种多效价活疫苗、其制备方法及应用. 中国发明专利. 申请号：200910054113.9

　　7张元兴, 王启要, 刘琴, 吴海珍, 肖婧凡. 迟钝爱德华氏菌天然弱毒株及其应用. 中国发明专利. 申请号：201210240313.5

　　8 王启要, 刘琴, 张元兴, 王亚敏, 吴海珍, 肖婧凡. 迟钝爱德华氏菌突变株及其应用, PCT国际专利: PCT/CN2012/076961

　　9 王启要, 王亚敏, 刘琴, 吴海珍, 肖婧凡, 张元兴. 鱼用多效价活疫苗及其应用, 中国发明专利. 申请号：201210492313.4

　　10 刘琴, 张元兴, 郑雁, 王启要. 一种抗鳗弧菌和迟钝爱德华氏菌的多效价活疫苗、相关表达载体及应用. 中国发明专利. 专利号：201010600524.6

　　11 肖婧凡, 张元兴, 刘和松, 王启要, 刘琴. 迟钝爱德华氏菌快速检测试纸条及快速检测方法与应用. 中国发明专利. 申请号：201010568826.X

　　12 吴海珍, 张萌, 李小勇, 沈斌兵, 王启要, 张元兴. 迟钝爱德华氏菌免疫保护性抗原、相关表达载体、疫苗和应用. 中国发明专利. 专利号：ZL201110095826.7

　　本科和研究生教学：

　　1. 生物科学专业大三第2学期课程，生物信息学，32学时，2学分，专业必修课；

　　2. 博士研究生专业课程：微生物细胞信号转导，32学时，2学分，专业选修课；

　　3. 2008级生物技术081班班导师；2012级生物技术121班班导师。