主要学术论文

　　1) Liang, X.G.*; Zhou, M.G.; Kato，K.; Asanuma, H.* Photoswitch nucleic acid catalytic activity by regulating topological structure with a universal supra-photoswitch. ACS Synthetic Biology 2013, http://dx.doi.org/10.1021/sb300120n

　　2) Kato, T.; Liang X.G.; Asanuma, H. Model of elongation of short DNA sequence by thermophilic DNA polymerase under isothermal conditions. Biochemistry. 2012, 51(40), 7846-7853.

　　3) Liang, X. G.;* Mochizuki, T.; Fujii, T.; Kashida, H.; Asanuma, H.* Design of an artificial functional nanomaterial with high recognition ability. Natural Computing, 2012, 11, 231-238.

　　4) Ito, H.; Urushihara, M.; Liang, X. G.;* Asanuma, H.* Improvement of RNAi activity and strand-selecivity of RISC formation by modified siRNA involving intercalators near 5'-termini. ChemBioChem, 2012, 13, 311-315.

　　5) Nishioka, H.; Liang, X.G.;* Kato, T.; Asanuma, H.* Photon-fueled DNA nanodevice carrying two different photoswitches. Angew. Chem. Int. Ed., 2012, 51, 1165-1168.

　　6) Liang, X. G.;* Fujioka, K.; Asanuma, H.* Nick Sealing by T4 DNA Ligase on a Modified DNA Template Tethering a Functional Molecule on D-Threoninol. Chem. Eur. J., 2011, 17, 10388-10396.

　　7) Asanuma, H.;* Toda, T.; Murayama, K.; Liang, X.G.; Kashida, H. Unexpectedly stable artificial duplex from flexible acyclic threoninol. J. Am. Chem. Soc., 2010, 132, 14702-14703.

　　8) Ito, H.; Liang, X.G.;* Nishioka, H.; Asanuma, H.* Construction of photoresponsive RNA for photoswitching RNA hybridization. Org. Biomol. Chem., 2010, 8, 5519-5524.

　　9) Tanaka, F.;* Mochizuki, T.; Liang, X.G.; Asanuma, H.; Tanaka, S.; Suzuki, K.; Kitamura, S.; Nishikawa, A.; Ui-Tei, K.; Hagiya, M. Robust and photo-controllable DNA capsules using azobenzenes. Nano Lett., 2010, 10, 3560-3565.

　　10) Hara, Y.; Fujii, T.; Kashida, H.; Sekiguchi, K.; Liang, X. G.; Niwa, K.; Takase, T.; Yoshida, Y.; Asanuma, H.* Coherent Quenching of a Fluorophore for the Design of a Highly Sensitive In-Stem Molecular Beacon. Angew. Chem. Int. Ed., 2010, 49, 5502-5506.

　　11) H. Kashida, K. Sekiguchi, X. G. Liang, H. Asanuma.* Accumulation of fluorophores into DNA duplexes to mimic the properties of quantum dots. J. Am. Chem. Soc., 2010, 132, 6223-6230.

　　12) Zhou, M.G.; Liang, X.G.*; Mochizuki, T.; Asanuma, H.* A light-driven DNA nanomachine for efficiently photoswitching RNA digestion. Angew. Chem. Int. Ed. 2010, 49, 2167-2170. (seleced as a hot paper, reported by Chemistry World (Royal Society of Chemistry), Highlighted in Nature 2010, 464, 10.)

　　13) Liang, X.G.;* Wakuda, R.; Fujioka, K.; Asanuma, H. Photoregulation of DNA transcription by using photoresponsive T7 promoters and clarification of its mechanism. FEBS J., 2010, 277, 1551-1561. (selected as the front cover)

　　14) Nishioka, H.; Liang, X.G.;* Asanuma, H. * Effect of the ortho modification of azobenzene on the photoregulatory efficiency of DNA hybridization and thermal stability of its cis-form. Chem. Eur. J., 2010, 16, 2054-2062.

　　15) Liang, X.G.; Nishioka, H.; Mochizuki, T.; Asanuma, H. An interstrand-wedged duplex composed of alternating DNA base pairs and covalently attached intercalators. J. Mater. Chem. 2010, 20, 575-581.

　　16) Kashida, H.; Takatsu, T.; Fujii, T.; Sekiguchi, K.; Liang, X.G.; Niwa, K.; Takse, T.; Yoshida, Y.; Asanuma, H. In-Stem molecular beacon containing a pseudo base pair of threoninol nucleotides for removal of background emission. Angew. Chem. Int. Ed. 2009, 48, 7044-7047.

　　17) Liang, X.G.; Mochizuki, T.; Asanuma, H. A supra-photoswitch involving sandwiched DNA base pairs and azobenzenes for light-driven nanostructures and nanodevices. Small, 2009, 15, 1761-1768. (Our work was used as the cover of issue 15.)

　　18) Kashida, H.; Liang, X.G.; Asanuma, H. Rational design of functional DNA with a non-ribose acyclic scaffold. Curr. Org. Chem., 2009, 13, 1065-1084.

　　19) Liang, X.G.; Takenaka, N; Nishioka, H.; Asanuma, H.* Molecular design for Reversing the photoswitching mode of turning ON and OFF DNA hybridization. Chem. Asian. J. 2008, 3, 553-560.

　　20) Liang, X.G.; Nishioka, H.; Takenaka, N; Asanuma, H.* A DNA nanomachine powered by light irradiation. ChemBioChem. 2008, 9, 702-705.

　　21) Nishioka, H.; Liang, X.G.; Kashida, H.; Asanuma, H.* 2?,6?-Dimethylazobenzene as an Efficient and Thermo-Stable Photo-Regulator for the Photoregulation of DNA Hybridization. Chem. Commun. 2007, 4354-4356.

　　22) Asanuma, H.; Liang, X.G.; Nishioka, H.; Matsunaga, D.; Liu, M.Z.; Komiyama, M. Synthesis of azobenzene-tethered DNA for reversible photo-regulation of DNA functions: hybridization and transcription. Nat. Protocol 2007, 2, 203-212.

　　23) Liang, X.G.; Kuhn, H.; Frank-Kamenetskii, M.D. Monitoring single-stranded DNA secondary structure formation by determining topological state of DNA catenanes. Biophys. J. 2006, 90, 2877-2889.

　　24) Liang, X.G.; Jensen, K.; Frank-Kamenetskii, M.D. Very efficient template/primer- independent DNA synthesis by thermophilic DNA polymerase in the presence of a thermophilic restriction endonuclease. Biochemistry 2004, 43, 13459-13466.

　　25) Liang, X.G.; Asanuma H.; Kashida, H.; Takasu, A.; Sakamoto, T.; Kawai, G.; Komiyama, M. NMR Study on the photoresponsive DNA tethering an azobenzene: Assignment of the absolute configuration of two diastereomers and structure determination of their duplex in the trans-form. J. Am. Chem. Soc. 2003, 125, 16408-16415.

　　26) Komiyama, M.; Ye, S.; Liang, X.G.; Yamamoto, Y.; Tomita, T.; Zhou, J.M.; Aburatani, H. PNA for one-base differentiating protection of DNA from nuclease and its use for SNPs detection. J. Am. Chem. Soc. 2003, 125, 3758-3762.

　　27) Liang, X.G.; Asanuma, H.; Komiyama, M. Photoregulation of DNA triplex formation by azobenzene. J. Am. Chem. Soc. 2002, 124, 1877-1883.

　　28) Asanuma, H.; Takarada, T.; Yoshida, T.; Tamaru, D.; Liang, X.G.; Komiyama, M. Enantioselecive incorporation of azobenzene into oligodeoxyribonucleotide for effective photoregulation of duplex formation. Angew. Chem. Int. Ed. 2001, 40, 2671-2673.

　　29) Asanuma, H.; Liang, X.G.; Yoshida, T.; Yamazawa, A.; Komiyama, M. Photocontrol of triple-helix formation by using azobenzene-bearing oligo(thymidine). Angew Chem. Int. Ed. 2000, 39, 1316-1319.

　　30) Yamazawa, A.; Liang, X.G.; Asanuma, H.; Komiyama, M. Photoregulation of DNA polymerase reaction by oligonucleotides bearing an azobenzene. Angew. Chem. Int. Ed. 2000, 39, 2356-2358.

　　31) Asanuma, H.; Ito, T.; Yoshida, T.; Liang, X.G.; Komiyama, M. Photoregulation of the formation and dissociation of a DNA-duplex by using the Cis-Trans isomerization of azobenzene. Angew. Chem. Int. Ed. 1999, 38, 2393-2395.

　　2、学术著作：

　　1) Wang, D.F.; Yu, L.Y.; Li, H.Y.; Zhang, B.; Wang, S.H.; Liang, X.G. Chapter 8 Minerals. Food Chemistry. 2012, Nova Science Publishers, Inc. New York. (English) ISBN 978-1-61942-125-7.

　　2) Asanuma, H. Liang, X.G. Design of photoresponsive DNA by use of cartridge-type artificial nucleotide, in “New trend of nucleic acid chemistry”, 2011, the chemical society of Japan, Kagaku-Dojin Publishing Company, INC. ISBN: 9784759813661.

　　3) Liang, X.G.; Kato, T. Asanuma, H. De Novo DNA Synthesis by DNA Polymerase. in “Bacterial DNA, DNA Polymerase and DNA Helicases.” 2009, Nova Science Publishers, Inc. (English) ISBN: 9781607410942.

　　4) Asanuma, H.; Liang, X.G. The Dynamic Photoregulation of DNA. in “Science and Technology of Supramolecule – from base to Innovation.” 2009, New Technology New Science (NTS) Press. (Japanese) ISBN: 979-4-86043-309-3.

　　5) Zhou, C.L; Liang, X.G.; Diao, X.H.; Yang, H.M; Zhang, T.Y.; Wang, S.R. Experiment Methods for the Synthesis of Fine Chemicals. 1998, China Petrochemical Press. (Chinese) ISBN 7-80043-718-3. 4-43.

　　专利申请：

　　1) Liang, X.G.; Asanuma, H.; Suzuki, M.; Kato, T. “Method for DNA amplification.” JP2009-156940 (JP2011010591-A).

　　2) Liang, X.G.; Asanuma, H.; Suzuki, M.; Kato, T. “Specific amplification of target DNA.” JP2009-145741 (JP2011000058-A).

　　3) Asanuma, H.; Liang, X.G.; Kashida, H.; Yoshida, Y.; Takase, T.; Niwa, K. “Oligonucleotide probes and their applications.” JP2008-17252 . (2008-285966, PCT/JP2009-061980, WO/2010/001902)

　　4) Asanuma, H.; Liang, X.G.; Kashida, H.; “Detection of target nucleic acids.” JP2009-298170. (2011-125824)

　　5) Asanuma, H.; Liang, X.G.; Kashida, H.; Hara Y. “Fluorescence probes and their applications.” JP2009-298160. (2011-135823)

　　6) Asanuma, H.; Liang, X.G.; Kashida, H.; Nishioka, H.; Fujii, T.; Ishikawa, T. “Modified oligonucleotides and their applications.” JP2010-194942.

　　7) Ye, S.; Yamamoto, Y.; Komiyama, M.; Liang, XG. “Non-labeled detection of SNPs using PNA and single stranded nuclease.” JP2004-033003.

　　8) Asanuma, H.; Liang, X.G.; Ito, Hiroshi. “Modified oligonucleotides and their applications in RNAi.” JP2011-?????.